* 1986/12/12 13:06:31, 1984/05/05 21:12:50, ex0: example organism * ecoli * escherichia coli (mostly k-12) note * # 1 * theodor escherich. died 1911. german physician * aerobic, gram-negative rod-shaped bacteria * family enterobacteriaceae * normally present in vertebrate intestines * - * microbiological reviews 44(1): 1-56 1980 bachmann and low * linkage map of escherichia coli k-12, edition 6 * - * genetic maps june 1982 vol 2 * stephen j. o'brien ed. * lab of viral carcinogenisis nci, nih * frederick, maryland 21701 note * minutes chromosome * ecoli * main chromosome of e. coli note * # 2 note * 0.00 * 100.00 gene * laci * lac repressor note * # 3 note * lac * 7.90 * + * 29 * 1111 gene piece * lac * lac i to lac z note * # 4 * lac i from farabaugh, nature 274,765 ('78) * pre-lac i from calos, nature 274,762 ('78) * lac z from maxam et al., unpublished, amino acid sequence * confirmed from zabin and fowler in "the operon", p89 * lacz sequence from: * a. kalnins, k. otto, u. ruther and b. mueller-hill * embo j. 2(4); 593-597 (1983) * lacy and lac a sequences form: * buechel et al. nature 238, 541 (feb 7, 1980) * - * two changes were noted between the original lacz sequence * and the new published one; 1332 (c -> t) and 1392 (t -> c). since these * changes do not alter the protein, they may be strain differences. * the new sequence at 1392 should be detectable because it is a hhai site. * - * the entire lac piece is now identical to the ecolac sequence * available through genbank. the only difference found upon * comparing the two sequences was at position -32; the g from the * original delila sequence has been replaced by the c found in * the genbank sequence. (5/3/84) note * 7.90 * linear * - * -49 * 5754 * linear * + * 29 * 49 dna * gtgaaaccagtaacgttatac dna piece chromosome organism